Biosensors

Heart of the matter

A microfluidic device developed in Singapore provides fast and selective detection of low levels of vascular disease biomarkers

Published online 14 April 2010

Fig. 1: The endothelial progenitor cell detection chip (inset) could be used in the assessment of plaque build-up in blood vessels (main image).

© 2010 Ji Hong Miao (inset)
© iStockphoto/Daft_Lion_Studio (main image)

The detection of endothelial progenitor cells (EPCs)—specific biomarkers for vascular diseases that exist at low concentrations in blood—is a time-consuming process that requires large blood-sample volumes from patients. To reduce patient discomfort and delay in vascular disease diagnosis, a team led by Yu Chen from the Institute of Microelectronics of A*STAR, Singapore, has developed a microfluidic device that rapidly detects low EPC levels in blood-cell samples1.

The researchers adapted a gold microelectrode array (MEA), previously employed in cell attachment studies2, for their EPC detection device. A unique configuration of horse-shoe shaped microfabricated electrodes concentrates EPCs at the center of other, disk-shaped electrodes through a technique called negative dielectrophoresis. Measuring the impedance between the horse-shoe shaped and disk electrodes allows the device to spot EPCs and determine their concentration.

“The electrode design combines both impedance detection and negative dielectrophoresis functions—which is different from approaches proposed by other researchers,” says Chen.

The researchers modified the array by depositing a layer of organosulfur molecules on the gold electrodes through self-assembly. Then, they fixed a microchamber comprised of a well flanked by two holes serving as fluid inlet and outlet to deliver blood samples onto this array. Finally, they tethered EPC-selective antibodies to the self-assembled monolayer to create EPC-specific MEA chips.

According to Chen, they needed antibodies that specifically bind EPCs because the target cells are mixed with peripheral blood mononuclear cells (PBMCs) in the sample. Therefore, they loaded the sample in the open microchamber and removed the non-specific PBMCs through a washing step while keeping the EPCs on the electrode.

EPCs assist the repair of blood vessel linings by differentiating into vascular endothelial cells. They also enhance the efficiency of stents—tubes inserted into blood vessels—to prevent the accumulation of plaque on arterial walls, which restricts blood flow to the heart. This microfluidic EPC detection system may be helpful for cardiologists to assess the need for stent implants in patients and determine if stents work properly (Fig. 1).

Chen and her co-workers are currently optimizing their sample preparation process to better isolate EPCs and PBMCs from blood. They are also investigating methods to further integrate separation and detection functions. “The final goal is to detect EPCs from [a] few drops of blood through sample preparation and detection functions,” says Chen. “The system will also have multiplexing electrodes with a simultaneous read out function, which is suitable for bedside applications.”

 

The A*STAR affiliated authors in this highlight are from the Institute of Microelectronics

References

  1. Ng, S.Y., Reboud, J., Wang, K.Y.P., Tang, K. C., Zhang, L., Wong, P., Moe, K.T., Shim, W. & Chen, Y. Label-free impedance detection of low levels of circulating endothelial progenitor cells for point-of-care diagnosis. Biosensors and Bioelectronics 25, 1095–1101 (2010). | article
  2. Chen, Y., Zhang, J., Wang, Y., Zhang, L., Reboud, J., Tang, K., Balasubramanian, N. Real-time monitoring approach: Assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix. Biosensors and Bioelectronics 23, 1390–1396 (2008). | article